Unexpected differences in the behavior of ovotransferrin at the air-water interface at pH 6.5 and 8.0. - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Journal of Colloid and Interface Science Année : 2011

Unexpected differences in the behavior of ovotransferrin at the air-water interface at pH 6.5 and 8.0.

Résumé

Adsorption of purified apo-ovotransferrin at the air-water interface was studied by ellipsometry, surface tension, polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS), and shear elastic constant measurements. No significant difference was observed between pH 6.5 and 8.0 as regards the final value of surface concentration and surface pressure. However at low concentration, a weak barrier to adsorption is evidenced at pH 6.5 and confirmed by PM-IRRAS measurements. At a pH where the protein net charge is negative (pH 8.0), the behavior of ovotransferrin at the air-water interface is more influenced by charge effects rather than bulk concentration effects. At this pH, the interface exhibits a low shear elastic constant and a spectral signature not usual for globular proteins.

Domaines

Biophysique

Dates et versions

hal-00711895 , version 1 (26-06-2012)

Identifiants

Citer

Cécile Le Floch-Fouéré, Stéphane Pezennec, Michel Pézolet, Jean-François Rioux-Dubé, Anne Renault, et al.. Unexpected differences in the behavior of ovotransferrin at the air-water interface at pH 6.5 and 8.0.. Journal of Colloid and Interface Science, 2011, 356 (2), pp.614-23. ⟨10.1016/j.jcis.2011.01.073⟩. ⟨hal-00711895⟩
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