Effect of temperature and time of storage on protein stability and anti-salmonella activity of egg white. - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Journal of Food Protection Année : 2010

Effect of temperature and time of storage on protein stability and anti-salmonella activity of egg white.

Sophie Réhault-Godbert
Florence Baron
  • Fonction : Auteur
  • PersonId : 1202779
Sandrine Mignon-Grasteau
Valérie Labas
M.T. Hincke
  • Fonction : Auteur
Y. Nys
  • Fonction : Auteur

Résumé

Hen egg white contains numerous molecules of interest for human health, including antimicrobial proteins. Little information is available concerning changes in the antimicrobial activity of egg white during storage; therefore, we analyzed the potential of egg white to inhibit growth of Salmonella enterica serovar Enteritidis following storage at 4, 20, or 37°C for 30 days prior to inoculation. Egg white displayed higher anti-Salmonella activity after a few days of storage at 20 and 37°C. The rate of increase in activity was more rapid and pronounced at the higher temperature. However, egg white stored at 20°C retained higher antimicrobial activity than that of egg white stored at 4 or 37°C, when the entire storage period is taken in consideration. In contrast, storage of egg at 37°C for more than 14 days reduced the bacteriostatic potential of egg white. Statistical analyses revealed a correlation between pH and the antimicrobial activity of egg white. Moreover, diminished antimicrobial activity was associated with degradation of ovalbumin and ovotransferrin, as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. However, the fluctuation in anti-Salmonella activity of egg white could not be related to any variation of trypsin-like, chymotrypsin-like, or gelatinolytic activities that potentially account for degradation of antimicrobial egg white proteins.
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Dates et versions

hal-00729488 , version 1 (07-09-2012)

Identifiants

  • HAL Id : hal-00729488 , version 1
  • PUBMED : 20828465

Citer

Sophie Réhault-Godbert, Florence Baron, Sandrine Mignon-Grasteau, Valérie Labas, Michel Gautier, et al.. Effect of temperature and time of storage on protein stability and anti-salmonella activity of egg white.. Journal of Food Protection, 2010, 73 (9), pp.1604-12. ⟨hal-00729488⟩
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