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Journal Articles Cryo Letters Year : 2010

How to start a programme on Rosa cryopreservation?


GenHort research activities are dealing with genetics of horticultural species and this unit is in charge of several collections of plant genetic resources. Some works are focused in increasing knowledge of our collections, studying the impact of domestication and selection on the genetic variability, defining strategies for an optimal management of these genetic resources. In order to secure the vegetatively propagated collections, studies on cryopreservation have been undertaken on Pelargonium since 1999. The main objective was to determine if shoot tip cryopreservation could play a role in the long term maintenance of healthy collections of vegetatively propagated plants. An efficient and reproducible protocol of cryopreservation with a droplet-vitrification procedure allowed obtaining good survival and regeneration rates. Soon, new activities will take place in the cryopreservation studies developed at GenHort. For an applied aim, cryopreservation of Malus and Pyrus, using a dormant bud protocol will be undertaken. A new program focused on the genus Rosa is just about to be initiated. The sub-genus Rosa comprises over 180 wild species, assigned in 10 sections, and plentiful of cultivars within 2 clusters: the old garden roses (20 groups) and the modern roses (13 groups). It presents a huge diversity regarding geographic origins, plant architecture, flowering physiology, environmental adaptation, morphological characters etc. Knowing the few reports previously published on Rosa cryopreservation, the present purpose is to define the scientific questions which can emerge and the best way to approach their solution It is the aim to set up three kinds of cryopreservation protocols (encapsulation-dehydration, droplet vitrification and dormant buds). The emerging questions would be: 1) is the tolerance to cryopreservation influenced by the genotype, 2) could the level of tolerance be related to the infrageneric diversity classification? The diversity within a collection of genetic resources could then be affected by the chosen conservation method.
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hal-00729532 , version 1 (07-09-2012)


  • HAL Id : hal-00729532 , version 1


Agnès Grapin. How to start a programme on Rosa cryopreservation?. Cryo Letters, 2010, 31 (1), 84 p. ⟨hal-00729532⟩
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