Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.). - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue TAG Theoretical and Applied Genetics Année : 2008

Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.).

Régine Delourme
N. Piel
  • Fonction : Auteur
R. Horvais
  • Fonction : Auteur
N. Pouilly
  • Fonction : Auteur
C. Domin
  • Fonction : Auteur
P. Vallee
  • Fonction : Auteur
Cyril Falentin
Maurice Renard
  • Fonction : Auteur
  • PersonId : 841683

Résumé

The most common and effective way to control phoma stem canker (blackleg) caused by Leptosphaeria maculans in oilseed rape (Brassica napus) is by breeding resistant cultivars. Specific resistance genes have been identified in B. napus and related species but in some B. napus cultivars resistance is polygenic [mediated by quantitative trait loci (QTL)], postulated to be race non-specific and durable. The genetic basis of quantitative resistance in the French winter oilseed rape 'Darmor', which was derived from 'Jet Neuf', was previously examined in two genetic backgrounds. Stable QTL involved in blackleg resistance across year and genetic backgrounds were identified. In this study, near isogenic lines (NILs) were produced in the susceptible background 'Yudal' for four of these QTL using marker-assisted selection. Various strategies were used to develop new molecular markers, which were mapped in these QTL regions. These were used to characterize the length and homozygosity of the 'Darmor-bzh' introgressed segment in the NILs. Individuals from each NIL were evaluated in blackleg disease field trials and assessed for their level of stem canker in comparison to the recurrent line 'Yudal'. The effect of QTL LmA2 was clearly validated and to a lesser extent, QTL LmA9 also showed an effect on the disease level. This work provides valuable material that can be used to study the mode of action of genetic factors involved in L. maculans quantitative resistance.

Dates et versions

hal-00729890 , version 1 (07-09-2012)

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Citer

Régine Delourme, N. Piel, R. Horvais, N. Pouilly, C. Domin, et al.. Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.).. TAG Theoretical and Applied Genetics, 2008, 117 (7), pp.1055-67. ⟨10.1007/s00122-008-0844-x⟩. ⟨hal-00729890⟩
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