Myristic acid increases $\Delta$6-desaturase activity in cultured rat hepatocytes
Résumé
In order to study the effects of saturated fatty acids on $\Delta$6-desaturase activity, rat hepatocytes in primary culture were incubated with lauric (C12:0), myristic (C14:0) or palmitic (C16:0) acids. After optimization, the standard in vitro conditions for the measurement of $\Delta$6-desaturase activity were as follows: 60 $\mu$mol*L-1$\alpha$-linolenic acid (C18:3n-3), reaction time of 20 min and protein content of 0.4 mg. Data showed that cell treatment with 0.5 mmol*L-1 myristic acid during 43 h specifically increased $\Delta$6-desaturase activity. This improvement, reproducible for three substrates of $\Delta$6-desaturase, i.e. oleic acid (C18:1n-9), linoleic acid (C18:2n-6) and $\alpha$-linoleic acid (C18:3n-3) was dose-dependent in the range 0.1-0.5 mmol*L-1 myristic acid concentration.
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