Investigating the impact of ovalbumin aggregate morphology on in vitro ovalbumin digestion using label-free quantitative peptidomics and multivariate data analysis - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Food Research International Année : 2014

Investigating the impact of ovalbumin aggregate morphology on in vitro ovalbumin digestion using label-free quantitative peptidomics and multivariate data analysis

Résumé

This study aimed to investigate how food structure, in the form of different ovalbumin aggregate morphologies, impacted the proteolysis of ovalbumin using an in vitro model that simulated digestion in the adult gastrointes- tinal tract. Four different aggregate morphologies were prepared by heating ovalbumin solution using different combinations of pH and ionic strength. Quantitative peptidomics (label-free) and multivariate data analysis of the resulting in vitro digests were performed. The 593 identified peptides were distributed in 6 homogeneous clusters based on the relative amount of peptide release from the different aggregate morphologies. Each cluster gatheredpeptideswithcommonphysicochemicalcharacteristics.Theresultssuggestthatpepticandchymotryp- tic cleavages were favored by aggregation regardless of the aggregate morphology, while tryptic cleavages were favored when ovalbumin aggregates were spherical-agglomerated. It is notable that even after extensive diges- tion, the initial aggregate morphology in fluenced the amount of each peptide released
Fichier non déposé

Dates et versions

hal-01189975 , version 1 (01-09-2015)

Identifiants

Citer

Kéra Nyemb, Julien Jardin, David Causeur, Catherine Guérin-Dubiard, Didier Dupont, et al.. Investigating the impact of ovalbumin aggregate morphology on in vitro ovalbumin digestion using label-free quantitative peptidomics and multivariate data analysis. Food Research International, 2014, 63, pp.192-202. ⟨10.1016/j.foodres.2014.03⟩. ⟨hal-01189975⟩
257 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More