The bacterial effector DspA/E is toxic in Arabidopsis thaliana and is required for multiplication and survival of fire blight pathogen - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Molecular Plant Pathology Année : 2013

The bacterial effector DspA/E is toxic in Arabidopsis thaliana and is required for multiplication and survival of fire blight pathogen

Résumé

The type III effector DspA/E is an essential pathogenicity factor of the phytopathogenic bacterium Erwinia amylovora. We showed that DspA/E was required for transient bacterial growth in nonhost Arabidopsis thaliana leaves, as an E.amylovora dspA/E mutant was unable to grow. We expressed DspA/E in A.thaliana transgenic plants under the control of an oestradiol-inducible promoter, and found that DspA/E expressed inplanta restored the growth of a dspA/E mutant. DspA/E expression in these transgenic plants led to the modulation by at least two-fold of the expression of 384 genes, mostly induced (324 genes). Both induced and repressed genes contained high proportions of defence genes. DspA/E expression ultimately resulted in plant cell death without requiring a functional salicylic acid signalling pathway. Analysis of A.thaliana transgenic seedlings expressing a green fluorescent protein (GFP):DspA/E fusion indicated that the fusion protein could only be detected in a few cells per seedling, suggesting the degradation or absence of accumulation of DspA/E in plant cells. Consistently, we found that DspA/E repressed plant protein synthesis when injected by E.amylovora or when expressed in transgenic plants. Thus, we conclude that DspA/E is toxic to A.thaliana: it promotes modifications, among which the repression of protein synthesis could be determinant in the facilitation of necrosis and bacterial growth.

Dates et versions

hal-01190557 , version 1 (01-09-2015)

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Citer

Alexandre A. Degrave, Manon Moreau, Alban Launay, Marie Anne Barny, Marie-Noelle Brisset, et al.. The bacterial effector DspA/E is toxic in Arabidopsis thaliana and is required for multiplication and survival of fire blight pathogen. Molecular Plant Pathology, 2013, 14 (5), pp.506 - 517. ⟨10.1111/mpp.12022⟩. ⟨hal-01190557⟩
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