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Poster De Conférence Année : 2015

Transport of food digesta and model particles in the small intestinal mucus

Résumé

The last boundary between ingested food and the gastrointestinal (GI) tract mucosa is the mucus layer. This highly complex viscoelastic medium has evolved to provide a robust barrier that traps and immobilises potentially hazardous particulates such as bacteria but still allows the passage of nutrients to the epithelial surfaces. However, the rules governing this selective barrier function, particularly in relation to transport of particulates, remain unknown. Recent studies suggest that surface properties of model nanoparticles can impact on colloidal transport in intestinal mucus (1). In particular, adsorption of bile salts (BS) to particles proved to significantly enhance their diffusion in the small intestinal mucus in vitro (2). The concentration of BS in the human small intestinal lumen can, however, vary greatly between the fasted and the fed states. There is also a significant difference in the overall concentration of BS between different age groups (e.g. infant vs. adult humans). In this study we used quantitative confocal microscopy to look at the transport characteristics in the porcine ex vivo (detached from the tissue) intestinal mucus of post-digestion food particulates such as yogurt digesta as well as lipid droplets that derived from in vitro digestion of sodium caseinate stabilised emulsions. Moreover, we used multiple-particle tracking technique to investigate the extent to which the concentration of BS can impact on the permeability of the mucus to model, sub-micron size particles such as latex beads, and their transport in this secretion. Finally, we investigated how the permeability and microstructure of the intact small intestinal mucus (i.e. attached to the mucosal tissue) may vary between specific locations on the tissue. Such insights will help support efforts to both engineer fabricated foods with enhanced nutritional quality required to fight the obesity epidemic, and to overcome the failure of many pharmaceutical and nutraceutical preparations to provide effective oral delivery of active compounds.
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Dates et versions

hal-01209787 , version 1 (02-10-2015)

Identifiants

  • HAL Id : hal-01209787 , version 1
  • PRODINRA : 282604

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Adam Macierzanka, A.R. Mackie, Didier Dupont, Francoise Nau, Olivia Ménard. Transport of food digesta and model particles in the small intestinal mucus. 4th International Conference of Food Digestion, Mar 2015, Naples, Italy. , 2015. ⟨hal-01209787⟩
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