Identification of a molecular dialogue between developing seeds of Medicago truncatula and seedborne xanthomonads. - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Botany Année : 2015

Identification of a molecular dialogue between developing seeds of Medicago truncatula and seedborne xanthomonads.

Emmanuel Terrasson
  • Fonction : Auteur
Armelle Darrasse
Karima Righetti
  • Fonction : Auteur
David Lalanne
  • Fonction : Auteur
Benoit Ly Vu
  • Fonction : Auteur
Sandra Pelletier
William Bolingue
  • Fonction : Auteur
Marie-Agnès Jacques
Olivier Leprince

Résumé

Plant pathogenic bacteria disseminate and survive mainly in association with seeds. This study addresses whether seeds are passive carriers or engage a molecular dialogue with pathogens during their development. We developed two pathosystems using Medicago truncatula with Xanthomonas alfalfae subsp. alfalfae (Xaa), the natural Medicago sp. pathogen and Xanthomonas campestris pv. campestris (Xcc), a Brassicaceae pathogen. Three days after flower inoculation, the transcriptome of Xcc-infected pods showed activation of an innate immune response that was strongly limited in Xcc mutated in the type three secretion system, demonstrating an incompatible interaction of Xcc with the reproductive structures. In contrast, the presence of Xaa did not result in an activation of defence genes. Transcriptome profiling during development of infected seeds exhibited time-dependent and differential responses to Xcc and Xaa. Gene network analysis revealed that the transcriptome of Xcc-infected seeds was mainly affected during seed filling whereas that of Xaa-infected seeds responded during late maturation. The Xcc-infected seed transcriptome exhibited an activation of defence response and a repression of targeted seed maturation pathways. Fifty-one percent of putative ABSCISIC ACID INSENSITIVE3 targets were deregulated by Xcc, including oleosin, cupin, legumin and chlorophyll degradation genes. At maturity, these seeds displayed decreased weight and increased chlorophyll content. In contrast, these traits were not affected by Xaa infection. These findings demonstrate the existence of a complex molecular dialogue between xanthomonads and developing seeds and provides insights into a previously unexplored trade-off between seed development and pathogen defence.

Dates et versions

hal-01392700 , version 1 (04-11-2016)

Identifiants

Citer

Emmanuel Terrasson, Armelle Darrasse, Karima Righetti, Julia Buitink, David Lalanne, et al.. Identification of a molecular dialogue between developing seeds of Medicago truncatula and seedborne xanthomonads.. Journal of Experimental Botany, 2015, 66 (13), pp.3737-52. ⟨10.1093/jxb/erv167⟩. ⟨hal-01392700⟩
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