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Poster De Conférence Année : 2018

Global gene-expression analysis of the response of Salmonella Enteritidis to egg-white exposure

Résumé

Egg white is a hostile medium for microorganisms due to its harsh physicochemical properties (alkaline pH and high viscosity), the nutritional restriction it imposes and its arsenal of antimicrobial molecules that may interact with bacteria in a synergistic way. Salmonella enterica serovar Enteritidis is the major pathogen responsible for egg borne infection in humans, due to its exceptional capability for survival under the harsh conditions encountered within egg white. However, above 42 ˚C, egg white exerts a much stronger bactericidal effect on S. Enteritidis than at lower temperatures, without full understanding of the responsible mechanism. Here we describe the first global-transcriptional analysis of the response of S. Enteritidis to egg white exposure under bactericidal conditions, providing a unique insight into egg white antibacterial activity. In order to enable this study, we used an “egg white model medium” (EWMM) already shown to be an appropriate mimic of egg white. Incubation of S. Enteritidis in EWMM above 42°C provoked a major transcriptional response (18.7% of genes affected at 45 min), indicative of major changes of S. Enteritidis physiology. Cell and envelope damage/stress responses were induced (degradation of unfolded protein, maintenance of cell integrity, and translation shutdown), energy metabolism shifted from respiration to fermentation, and micronutrient provision was enhanced (related to iron and biotin restriction). Our investigation of the global expression response of S. Enteritidis to egg white provides a complete genome-wide view of the complex physiological response of this pathogen to egg white-induced bacterial destruction under specific conditions.
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Dates et versions

hal-01901474 , version 1 (22-10-2018)

Identifiants

  • HAL Id : hal-01901474 , version 1
  • PRODINRA : 450012

Citer

Florence Baron, S. Bonnassié, Maria Alabdeh, Marie-Françoise Cochet, Catherine Guérin-Dubiard, et al.. Global gene-expression analysis of the response of Salmonella Enteritidis to egg-white exposure. International Egg Symposium, Oct 2018, Kyoto, Japan. , 2018. ⟨hal-01901474⟩
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