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Poster De Conférence Année : 2019

Impact of human milk pasteurization on digestive kinetics and intestinal lipid absorption using a combination of in vitro models

Résumé

Introduction Human milk is a complex biological fluid recognized as the gold standard in neonatal nutrition, especially for preterm infants since it supports growth and brain development. Donor human milk, pasteurized for safety reasons, is the first option to feed preterm infants when mothers’ own milk is not available. Objective The aim of the present study was to determine the impact of breastmilk pasteurization on preterm gastrointestinal digestion and to evaluate its effect on lipid absorption. Methodology Kinetics of digestion and structural desintegration of pasteurized human milk (PHM) and raw human milk (RHM) were evaluated using a static in vitro digestion model that we adapted to simulate preterm digestion ability. Caco-2 /TC7 cells, an intestinal absorption model, were subsequently incubated with diluted PHM vs RHM-digestion media. Main findings During in vitro digestion, proteolysis was evaluated by SDS-PAGE followed by densitometry. Lactoferrin proteolysis was significantly (p<0.05) higher in PHM than in RHM in the gastric and intestinal phases with 26±4 % and 53±18% of intact lactoferrin remaining at 60 min of intestinal digestion for PHM and RHM, respectively. β-casein was less susceptible to pepsinolysis in PHM, with 78±3 % remaining at 60 min of gastric digestion vs. 37±4% for RHM, but less than 5% remained at 60 min of intestinal digestion for both milks. The lipolysis kinetics, determined by thin layer chromatography, was not significantly different between RHM and PHM. In Caco-2 cells after 16h of incubation, cell monolayer integrity assesses by occludine immunostaining was unaffected and intracellular lipid droplets could be visible using Oil Red O staining. Triglyceride secretion measured in the basolateral medium seems to be higher for RHM than PHM, supporting further exploration of the gene expression involved in intestinal lipid absorption.
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Dates et versions

hal-02100438 , version 1 (02-06-2020)

Identifiants

  • HAL Id : hal-02100438 , version 1
  • PRODINRA : 467562

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Olivia Ménard, Amélie Deglaire, Didier Dupont, J. Picaud, Armelle Penhoat, et al.. Impact of human milk pasteurization on digestive kinetics and intestinal lipid absorption using a combination of in vitro models. 6.International Conference on Food Digestion, Apr 2019, Grenade, Spain. , 2019. ⟨hal-02100438⟩
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