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Poster De Conférence Année : 2019

Static in vitro digestion tests to assess allergenic risk of novel proteins

Résumé

Introduction Evaluating the gastrointestinal fate of proteins is paramount to assess whether they are safe to consume. The resistance of proteins to digestion may play a role in determining their allergenic potential since incomplete digestion may cause undesired immune responses via sensitisation in the duodenum. In order to evaluate the digestibility of proteins, in vitro protocols seem appropriate when ethical constraints hinder in vivo studies. The current in vitro digestion model used by the European Food Safety Authority (EFSA Journal 2017, 15, 4862) to assess allergenicity of proteins is the pepsin resistant test. This uses harsh gastric conditions of acidity and enzyme concentration that would mimic the end of gastric emptying (late phase) or fasted state in human adults. A more realistic approach considering other relevant conditions in healthy adults (early phase or fed state), or impaired digestion (e.g. infants) may provide useful information on how the combined effect of pH and enzyme concentration affects protein digestibility. Objective Our current project contracted by EFSA aims looking at the combination of three different static in vitro digestion models to evaluate the resistance of proteins to digestion by gastrointestinal enzymes that could sensitise and eventually trigger an allergic reaction. The main objective is the development and validation of a robust in vitro digestion protocol for purified proteins consistently reproducible across different laboratories. Methodology Comparison of an infant, early phase adult, and late phase adult model is being applied on a panel of allergen/non-allergen proteins from animal and vegetal origin. The proteolysis rate and extent is determined with SDS-PAGE and LCMS in order to detect persistent intact protein and hydrolysis products larger 9 amino acids. Main findings Differences in the kinetics of proteolysis may be found across models for proteins that are not pepsin resistant. Conclusion This project highlights the importance of a multi-test protocol to assess protein digestibility.
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Dates et versions

hal-02100440 , version 1 (15-04-2019)

Identifiants

  • HAL Id : hal-02100440 , version 1
  • PRODINRA : 467567

Citer

A. Torcello-Gomez, A. Mackie, Didier Dupont, Julien Jardin, Amélie Deglaire, et al.. Static in vitro digestion tests to assess allergenic risk of novel proteins. 6.International Conference on Food Digestion, Apr 2019, Grenade, Spain. , 2019. ⟨hal-02100440⟩
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