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Article Dans Une Revue Journal of the Science of Food and Agriculture Année : 2018

Investigation of secondary structure evolution of micellar casein powder upon aging by FTIR and SRCD: Consequences on solubility

Résumé

Background Synchrotron Radiation Circular Dichroism (SRCD) and Fourier Transform Infrared (FTIR) spectroscopy were used to examine the conformation evolution of micellar casein (MC) powder during storage and to determine whether the spectral changes could be related to their solubility evolution. Results A loss in intensity of SRCD spectra as a function of storage time has been observed. Quantification of secondary structures revealed losses of α-helix content during storage. Moreover, a redshift of Amide I band in the FTIR spectrum was demonstrated during the storage and was interpreted as a rearrangement of the secondary structure of the protein, which is in line with the SRCD results. The qualitative results obtained by FTIR clearly support the quantitative evolution of the secondary structure obtained by the analysis of SRCD spectra. Principal component analysis (PCA) of FTIR spectra permits a good separation of samples according to the storage time. PCA shows that the evolution of secondary structures and solubility loss are closely linked. Conclusion With the quantitative data provided by SRCD spectra, it was established that, whatever the storage conditions, a unique curve exists between loss of α-helix content and loss in solubility, showing that loss of α-helix content is a marker of solubility loss for the MC powders studied.
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Dates et versions

hal-02621245 , version 1 (26-05-2020)

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Sarah Nasser, Alain Hédoux, Alexandre A. Giuliani, Cécile Le Floch-Fouéré, Véronique Santé-Lhoutellier, et al.. Investigation of secondary structure evolution of micellar casein powder upon aging by FTIR and SRCD: Consequences on solubility. Journal of the Science of Food and Agriculture, 2018, 98 (6), pp.2243-2250. ⟨10.1002/jsfa.8711⟩. ⟨hal-02621245⟩
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