Nitrogen supply exerts a major/minor switch between two QTLs controlling <em>Plasmodiophora brassicae</em> spore content in rapeseed - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Plant Pathology Année : 2018

Nitrogen supply exerts a major/minor switch between two QTLs controlling Plasmodiophora brassicae spore content in rapeseed

Résumé

Clubroot, caused byPlasmodiophora brassicae, is a worldwide disease affectingBrassica. Until now, the detection ofgenetic factors (QTLs) implicated in clubroot resistance has been based on estimates of disease index. However, as theamount of resting spores released in soil after club disintegration influences clubroot epidemics and resistance-breakingdynamics, its genetic control may deserve specific attention. In a previous report, it was shown that nitrogen fertiliza-tion modulated quantitative partial resistance toward clubroot symptom development in rapeseed. The present workaimed to identify genetic factors involved in the control of resting spore production and to assess their regulation bynitrogen supply. A flow cytometer method was adapted for rapidly estimating resting spore content in a large series ofsamples. Linkage analysis was conducted to detect QTLs implicated in resting spore production in aBrassica napusdoubled haploid progeny from the cross Darmor-bzh9Yudal. DH lines inoculated with theP. brassicaeisolate eHwere grown under low- and high-nitrogen supply. Under low-nitrogen conditions, resting spore production was reducedcompared to high-nitrogen conditions, regardless of genotypes. Genetic architecture controlling resting spore produc-tion and clubroot symptom development was similar. Under high-nitrogen conditions, resting spore production wascontrolled by one major QTL (C09a) and a few small-effect QTLs. By contrast, two major QTLs (C02 and C09a) con-trolled resting spore production under low-nitrogen conditions. This work highlighted a large see-saw effect betweenthe relative contribution of the C09a QTL (high effect under high-nitrogen conditions) and the C02 QTL (high effectunder low-nitrogen conditions), with possible implications in resistance breeding
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Dates et versions

hal-02625390 , version 1 (26-05-2020)

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Yoann Aigu, Anne Laperche, Jean Mendes, Christine Lariagon, Solenn Guichard, et al.. Nitrogen supply exerts a major/minor switch between two QTLs controlling Plasmodiophora brassicae spore content in rapeseed. Plant Pathology, 2018, 67 (7), pp.1574-1581. ⟨10.1111/ppa.12867⟩. ⟨hal-02625390⟩
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