Effect of nutrient restriction on mammary cell turnover in lactating dairy cows - Institut Agro Rennes-Angers Accéder directement au contenu
Poster De Conférence Année : 2010

Effect of nutrient restriction on mammary cell turnover in lactating dairy cows

Résumé

The aim of the study was to investigate the effects of nutrient restriction on mammary cell turnover in lactating dairy cows. We used 15 Holstein x Normande crossbred dairy cows, divided into 2 groups submitted to 2 feeding levels. From calving to wk 11 postpartum, the cows were fed a total mixed ration composed either of 55% maize silage, 15% alfalfa hay and 30% concentrate (High-group, N=7) or of 60% grass silage and 40% hay (Low-group, N=8). Cows were milked twice daily. Milk yield (MY) and composition were measured. After 11 wk of lactation, cows were slaughtered and mammary glands were removed and weighed. The mammary DNA concentration was measured in order to measure the total amount of DNA in the mammary gland and to estimate the total number of mammary cells. Expression of proteins involved in proliferation and cell death were evaluated on mammary tissue by real-time qPCR, Western Blotting and immunohistochemical staining. Low-group cows had lower 11-week average daily MY from calving to slaughter than High-group cows (20.5 kg/d vs. 34.5, P<0.001). At the mammary tissue level, the total number of mammary cells and the size of the mammary acini were lower (-20% P<0.002, and – 55 % P<0.05, respectively) in the Low- group compared to the High-group. Mammary cell proliferation or apoptosis didn’t seem to be modified at the time of slaughtering but a remodelling of the extracellular matrix was observed.
Fichier principal
Vignette du fichier
Abt_ISEP2010_DESSAUGE_1.pdf (58.58 Ko) Télécharger le fichier
Abt_ISEP2010_DESSAUGE_2.doc (26.5 Ko) Télécharger le fichier
Poster ISEP_3.pdf (156.46 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02754233 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-02754233 , version 1
  • PRODINRA : 349165

Citer

Frederic Dessauge, Vanessa Lollivier, Erwan Cutullic, Catherine Disenhaus, Sarah Barbey, et al.. Effect of nutrient restriction on mammary cell turnover in lactating dairy cows. 3. EAAP International Symposium on Energy and Protein Metabolism and Nutrition, Sep 2010, Parme, Italy. Wageningen Academic Publishers, Publication - European Association for Animal Production (EAAP), 127, 2010, Energy and protein metabolism and nutrition. ⟨hal-02754233⟩
21 Consultations
4 Téléchargements

Partager

Gmail Facebook X LinkedIn More