Involvement of MtABI5 in the acquisition of desiccation tolerance in Medicago truncatula seeds and seedlings: characterization of potential new targets - Institut Agro Rennes-Angers Accéder directement au contenu
Poster De Conférence Année : 2014

Involvement of MtABI5 in the acquisition of desiccation tolerance in Medicago truncatula seeds and seedlings: characterization of potential new targets

Résumé

To identify the molecular processes and signaling pathways regulating desiccation tolerance (DT) in plant seeds, we characterized the transcriptomes of developing seeds before and after the acquisition of DT and compared these data with the transcriptomes obtained from germinated seeds that were desiccation sensitive and germinated seeds that were rendered tolerant after an osmotic treatment at -1.7 MPa. This comparative analysis provided a list of genes whose transcript levels increased and decreased in relation to DT. One of the transcription factors that was identified in this list was ABSICSIC ACID INSENSITIVE 5 (MtABI5), a bZIP family transcription factor. Abscisic acid (ABA) is a plant hormone that regulates development and induces tolerance against water stress. ABI5 is suggested to play a key role in the regulation of seed germination and postgerminative growth arrest by ABA but its precise role in DT remains unclear. In the legume seeds M. truncatula, characterization of Mtabi5 Tnt1 insertion mutants showed that DT could no longer be re-established in the radicles by the osmotic treatment at -1.7 MPa. However, Mtabi5 seeds did acquire their DT during development suggesting that redundant regulatory mechanisms are present or MtABI5 may act predominantly in the re-establishment of DT after germination rather than during development. To identify potential targets of MtABI5, a transcriptome analysis of germinated radicles of MtABI5 Tnt1 mutants was performed before and after osmotic treatment. Comparison of differentially expressed genes in Mtabi5 with wild type seeds showed that MtABI5 seems to act predominantly as a repressor rather than an activator of gene expression in the re-establishment of DT. Enrichment analysis using Gene Ontology shows that down regulated genes are involved in processes known to be related to DT in seeds such as cell division, growth and cell wall modification. In addition, our data suggest that defenses against biotic stress (LRR protein) are also repressed at the DT stage. To the best of our knowledge, such cross-talk has not been suggested before.

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hal-03226311 , version 1 (14-05-2021)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Julia Zinsmeister, Emmanuel Terrasson, Julia Buitink, Karima Righetti, Benoît Ly Vu, et al.. Involvement of MtABI5 in the acquisition of desiccation tolerance in Medicago truncatula seeds and seedlings: characterization of potential new targets. New Frontiers in Anhidrobiosis, Mar 2014, Pornichet, France. ⟨10.13140/RG.2.2.14923.92961⟩. ⟨hal-03226311⟩
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