Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by Bacillus cereus on Stainless Steel Surfaces in Food Processing Facilities - Institut Agro Rennes-Angers Accéder directement au contenu
Article Dans Une Revue Pathogens Année : 2022

Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by Bacillus cereus on Stainless Steel Surfaces in Food Processing Facilities

Clarisse Techer
  • Fonction : Auteur
Sophie Jan
Noël Grosset
  • Fonction : Auteur
  • PersonId : 1179098

Résumé

The Bacillus cereus (B. cereus) group is a widespread foodborne pathogen with a persistent ability to form biofilm, and with inherent resistance to traditional treatment in the food industry. Bacteriophages are a promising biocontrol agent that could be applied to prevent or eliminate biofilms formation. We have described, in this study, the isolation from sewage samples and preliminary characterization of bacteriophages that are active against the B. cereus group. The effectiveness of phage treatment for reducing B. cereus attachment and biofilms on stainless steel surfaces has been also assessed using three incubation periods at different titrations of each phage. Out of 62 phages isolated, seven showed broad-spectrum lytic action against 174 B. cereus isolates. All selected phages appeared to be of the Siphoviridae family. SDS-PAGE proved that two phages have a similar profile, while the remainder are distinct. All isolated phages have the same restriction pattern, with an estimated genome size of around 37 kb. The isolated bacteriophages have been shown to be effective in preventing biofilm formation. Reductions of up to 1.5 log10 UFC/cm2 have been achieved, compared to the untreated biofilms. Curative treatment reduced the bacterial density by 0.5 log10 UFC/cm2. These results support the prospect of using these phages as a potential alternative strategy for controlling biofilms in food systems
Fichier principal
Vignette du fichier
pathogens-11-00872.pdf (5.04 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03829993 , version 1 (26-10-2022)

Licence

Paternité

Identifiants

Citer

Maroua Gdoura-Ben Amor, Antoine Culot, Clarisse Techer, Mousa Alreshidi, Mohd Adnan, et al.. Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by Bacillus cereus on Stainless Steel Surfaces in Food Processing Facilities. Pathogens, 2022, 11, pp.872. ⟨10.3390/pathogens11080872⟩. ⟨hal-03829993⟩
33 Consultations
25 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More