Cryopreservation of Pelargonium apices by droplet-vitrification. - Institut Agro Rennes-Angers Access content directly
Journal Articles Cryo Letters Year : 2008

Cryopreservation of Pelargonium apices by droplet-vitrification.

A. Gallard
  • Function : Author
B. Panis
  • Function : Author
N. Dorion
  • Function : Author
R. Swennen
  • Function : Author
Agnès Grapin


The droplet-vitrification method was adapted to Pelargonium apices by optimizing the duration of the loading solution (LS) as well as the plant vitrification solution 2 (PVS2). The excised apices were dehydrated in two steps (20 min in LS and 15 min in PVS2) and then immersed directly in liquid nitrogen (LN). After thawing and unloading in the recovery solution at room temperature for 15 min, apices were plated onto semi-solid Murashige and Skoog medium. This simple protocol without any pretreatment was successfully applied to eight cultivars with a survival level ranging between 55.6 - 96.2 percent and a regrowth level between 9.1 and 70.6 percent. These results prove the feasibility of the long-term storage of Pelargonium germplasm through cryopreservation.
Not file

Dates and versions

hal-00729848 , version 1 (07-09-2012)


  • HAL Id : hal-00729848 , version 1
  • PUBMED : 18754064


A. Gallard, B. Panis, N. Dorion, R. Swennen, Agnès Grapin. Cryopreservation of Pelargonium apices by droplet-vitrification.. Cryo Letters, 2008, 29 (3), pp.243-51. ⟨hal-00729848⟩
47 View
1 Download



Gmail Facebook Twitter LinkedIn More