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Article Dans Une Revue Applied Microbiology and Biotechnology Année : 2020

Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying

Philippe Blanc

Résumé

Propionibacterium freudenreichii is a beneficial bacterium widely used in food as a probiotic and as a cheese-ripening starter. In these different applications, it is produced, dried, and stored before being used. Both freeze-drying and spray-drying were considered for this purpose. Freeze-drying is a discontinuous process that is energy-consuming but that allows high cell survival. Spray-drying is a continuous process that is more energy-efficient but that can lead to massive bacterial death related to heat, osmotic, and oxidative stresses. We have shown that P. freudenreichii cultivated in hyperconcentrated rich media can be spray-dried with limited bacterial death. However, the general stress tolerance conferred by this hyperosmotic constraint remained a black box. In this study, we modulated P. freudenreichii growth conditions and monitored both osmoprotectant accumulation and stress tolerance acquisition. Changing the ratio between the carbohydrates provided and non-protein nitrogen during growth under osmotic constraint modulated osmoprotectant accumulation. This, in turn, was correlated with P. freudenreichii tolerance towards different stresses, on the one hand, and towards freeze-drying and spray-drying, on the other. Surprisingly, trehalose accumulation correlated with spray-drying survival and glycine betaine accumulation with freeze-drying. This first report showing the ability to modulate the trehalose/GB ratio in osmoprotectants accumulated by a probiotic bacterium opens new perspectives for the optimization of probiotics production.

Domaines

Bactériologie
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Dates et versions

hal-02492112 , version 1 (26-02-2020)

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Floriane Gaucher, Houem Rabah, Koffigan Kponouglo, Sylvie Bonnassie, Sandrine Pottier, et al.. Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying. Applied Microbiology and Biotechnology, 2020, 104 (7), pp.3145-3156. ⟨10.1007/s00253-020-10425-1⟩. ⟨hal-02492112⟩
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